Nisha Joseph
Post Graduate and Research Department of Botany, Catholicate College Pathanamthitta, Kerala, India.
A. S. Deepthi
Post Graduate and Research Department of Botany, Catholicate College Pathanamthitta, Kerala, India.
Gokul G. Nair
Post Graduate and Research Department of Botany, Catholicate College Pathanamthitta, Kerala, India.
Merin Grace Jiji
Post Graduate and Research Department of Botany, Catholicate College Pathanamthitta, Kerala, India.
In this post, we present a brief overview of our recently published book chapter titled “Evaluation of α-Amylase Inhibitory Activity of Aqueous Extracts of Selected Plants”
The α-amylase inhibitory activity of five plant extracts (Aerva lanata, Emblica officinalis, Momordica charantia, Musa paradisiaca, and Psidium guajava) was evaluated at concentrations ranging from 10, 20, 40, 60, 80 and 100 μg/mL. Metformin was used as a standard antidiabetic drug for comparison. The results revealed a concentration-dependent increase in α-amylase inhibitory activity for all plant extracts, with Momordica charantia consistently demonstrating the highest inhibitory activity across all concentrations tested.
At the lowest concentration (10 μg/ml), Momordica charantia exhibited a remarkable inhibition of 66.33 ± 0.88%, significantly overtaking all other extracts (P < 0.001). This trend persisted with increasing concentrations, reaching 95.00 ± 0.58% inhibition at 100 μg/ml. The inhibition profile of Momordica charantia was statistically distinct across all concentrations, indicating its superior efficacy in suppressing α-amylase activity.
Emblica officinalis showed moderate inhibition, beginning at 14.33 ± 0.33% at 10 μg/ml and increasing steadily to 57.00 ± 0.57% at 100 μg/ml. Its performance was comparable to the standard drug Metformin, which ranged from 26.33 ± 0.33% to 58.33 ± 0.33% inhibition across the concentration series. Notably, at 100 μg/ml, Emblica officinalis and Metformin exhibited statistically similar inhibition levels, suggesting potential therapeutic relevance.
Musa paradisiaca demonstrated intermediate activity, with inhibition values ranging from 33.00 ± 0.57% at 10 μg/ml to 53.00 ± 0.57% at 100 μg/ml. Although its efficacy was lower than that of Emblica officinalis and Metformin at higher concentrations, it maintained consistent inhibition across the gradient.
Leave a Reply